Question 19
In an alkaline medium, potassium ferricyanide oxidizes hemoglobin to methemoglobin. Further reaction with potassium cyanide produces cyanmethemoglobin which has a maximum absorbance at 540 nm. Color intensity is proportional to total hemoglobin concentration, which is how the hemoglobin levels are measured.
Hematology
In the hemoglobin methodology using potassium ferricyanide the following measurable reaction occurs:
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carboxhemoglobin is formed
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iron remains in the ferrous state
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ferrous iron is oxidized to ferric iron to form methemoglobin
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acid hematin is formed
